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GrainGenes Marker Report: Bmac0067

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Locus	Bmac0067
Type	Microsatellite
Synonym	Synonym Bmac67
Chromosome	3H
Map	Barley, B73xCPI-3H 46.1 [ Show all 15 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.FHB_3H_4 QFhs.umn-3H.1
Map Data	Barley, Abiotic QTL Consensus [ Show all 15 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0067 Bmac67
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys,Cebada Capa x SusPtrit Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Oregon Wolfe Dom x Rec; Cebada Capa x SusPtrit Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant, Igri x Franka, Lina x H. spontaneum Insert contains repeat (AC)18 Polymorphic Information Content (PIC) = 0.82 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac67
Probe for Locus	Bmac67
Locus	Bmac0067 Bmac67
Synonym	Other name Bmac0067
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)18 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.82
Type	PCR SSR
PCR primers	5' AACGTACGAGCTCTTTTTCTA 3' 5' ATGCCAACTGCTTGTTTAG 3'
SSR size	171
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare