GrainGenes Marker Report: Bmac0090
Locus
Bmac0090
Type
Microsatellite
Synonym
Synonym Bmac90
Chromosome
1H
Map
Barley, B73xCPI-1H 52.3
[ Show all 7 ]
[ Show Nearby Loci ]
QTL with Locus Marker
MetaQ.FHB_1H_4 MetaQ.HD_1H_1 QFhs.umn-1H QHD.umn-1H
Map Data
[ Hide all but 1 of 7 ] Barley, B73xCPI Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, Integrated, Marcel 2009 Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
Bmac0090 Bmac90
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Possible Orthologs
Bmac90
Probe for Locus
Bmac90
Locus
Bmac0090 Bmac90
Synonym
Other name Bmac0090
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)20 Quality = 2: Single product, relatively strong stutter bands
Type
PCR SSR
PCR primers
5' ACATCAACCCTCCTGCTC 3'
5' CCGCACATAGTGGTTACATC 3' ACATCAACCCTCCTGCTC
CCGCACATAGTGGTTACATC
SSR size
221
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmac0090
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