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GrainGenes Marker Report: Bmac0154

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Locus
Bmac0154
Type
Microsatellite
Synonym
SynonymBmac154
Chromosome
1H
Map
[ Hide all but 1 of 8 ]
Barley, B73xCPI-1H68.8
Barley-ConsensusFHB-1H92.5
Hordeum-Consensus2005-SNP-1H91.513
Hordeum-Consensus2007-SSR-1H87.83
Hordeum-LiHs-SNP-1H82.77
Hordeum-LxHs-1H81
Hordeum-QTLConsensus-Abiotic-1H91.51
Hordeum-QTLConsensus-Agronomic-1H91.51
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Map Data
Barley, Abiotic QTL Consensus
[ Show all 8 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Bmac0154
Bmac154
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum
Insert contains repeat (AT)19/(AC)6
Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmac154


Probe for Locus
Bmac154
Locus
Bmac0154
Bmac154
Synonym
Other nameBmac0154
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AT)19/(AC)6
Quality = 1: Single product, only weak stutter bands
Null in H. spontaneum Canada Park.
Type
PCR
SSR
PCR primers
5' CTGGGTGATGAATAGAGTTTC 3'
5' TATTCTTCAAAAGATGTTCTGC 3'
SSR size
130
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare