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GrainGenes Marker Report: Bmac0186

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Locus	Bmac0186
Type	Microsatellite
Synonym	Synonym Bmac186
Chromosome	4H
Map	[ Hide all but 1 of 12 ] Barley, B73xCPI-4H 60.4 Barley-ConsensusFHB-4H 75.2 Hordeum-Consensus2005-SNP-4H 66.668 Hordeum-Consensus2007-SSR-4H 63.89 Hordeum-LxHs-4H 45 Hordeum-OWB-2004-4H 63.8 Hordeum-OWB-4H 69.6 Hordeum-OWB-SNP-4H 69.74 Hordeum-QTLConsensus-Abiotic-4H 66.67 Hordeum-QTLConsensus-Agronomic-4H 66.67 Hv-Consensus2006-Marcel-4H 63.89 Hv-Integrated2009-4H 73.4
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 12 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0186 Bmac186
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	Bmac186 Bmac186-4H
Probe for Locus	Bmac186
Locus	Bmac0186 Bmac186-4H Bmac186
Synonym	Other name Bmac0186
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)7 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' GTTGTTACGTGTGTGTCTGC 3' 5' CGCAACCTTACATCAACAC 3'
SSR size	129
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare