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GrainGenes Marker Report: Bmac0186

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Locus	Bmac0186
Type	Microsatellite
Synonym	Synonym Bmac186
Chromosome	4H
Map	Barley, B73xCPI-4H 60.4 [ Show all 12 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 12 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, LxHs Barley, OWB Barley, OWB, 2004 Barley, OWB, SNP
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0186 Bmac186
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	Bmac186 Bmac186-4H
Probe for Locus	Bmac186
Locus	Bmac0186 Bmac186-4H Bmac186
Synonym	Other name Bmac0186
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)7 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' GTTGTTACGTGTGTGTCTGC 3' 5' CGCAACCTTACATCAACAC 3'
SSR size	129
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare