GrainGenes Marker Report: Bmac0186
Locus
Bmac0186
Type
Microsatellite
Synonym
Synonym Bmac186
Chromosome
4H
Map
Barley, B73xCPI-4H 60.4
[ Show all 12 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 12 ]
Species
Hordeum spontaneum Hordeum vulgare
Probe
Bmac0186 Bmac186
Reference
Remark
[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Oregon Wolfe Dom x Rec Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant, Lina x H. spontaneum Insert contains repeat (AC)7 Polymorphic Information Content (PIC) = 0.6 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmac186 Bmac186-4H
Probe for Locus
Bmac186
Locus
Bmac0186 Bmac186-4H Bmac186
Synonym
Other name Bmac0186
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)7 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.6
Type
PCR SSR
PCR primers
5' GTTGTTACGTGTGTGTCTGC 3'
5' CGCAACCTTACATCAACAC 3'
SSR size
129
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmac0186
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|