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GrainGenes Marker Report: Bmac0209

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Locus	Bmac0209
Type	Microsatellite
Synonym	Synonym Bmac209
Chromosome	3H
Map	Barley, B73xCPI-3H 46.1 [ Show all 17 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 18 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0209 Bmac209
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	[ Hide all but 1 of 8 ] Locus position not reported in Cebada Capa x SusPtrit map Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L94 x Vada, Vada x SusPtrit, Oregon Wolfe Barleys, Cebada Capa x SusPtrit Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Vada x SusPtrit; Cebada Capa x SusPtrit Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, SusPtrit x Vada, OWBrecessive x OWBdominant, Igri x Franka, Lina x H. spontaneum Insert contains repeat (AC)13 Polymorphic Information Content (PIC) = 0.64 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac209
Probe for Locus	Bmac209
Locus	Bmac0209 Bmac209
Synonym	Other name Bmac0209
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)13 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' CTAGCAACTTCCCAACCGAC 3' 5' ATGCCTGTGTGTGGACCAT 3'
SSR size	176
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare