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GrainGenes Marker Report: Bmac0213

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Locus	Bmac0213
Type	Microsatellite
Synonym	Synonym Bmac213
Chromosome	1H
Map	Barley, B73xCPI-1H 21.3 [ Show all 13 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QHea.pil-1H.2
Map Data	[ Hide all but 1 of 13 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, AxHs/QTL Barley, B73xCPI Barley, Cali-sib x BowmanBC, QTL Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, Integrated, Marcel 2009 Barley, Lina x H.spont, SNP Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0213 Bmac213
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmac213
Probe for Locus	Bmac213
Locus	Bmac0213 Bmac213
Synonym	Other name Bmac0213
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ATGGATGCAAGACCAAAC 3' 5' CTATGAGAGGTAGAGCAGCC 3' ATGGATGCAAGACCAAAC CTATGAGAGGTAGAGCAGCC
SSR size	168
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare