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GrainGenes Marker Report: Bmac0224

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Locus	Bmac0224
Type	Microsatellite
Synonym	Synonym Bmac224
Chromosome	7H
Map	Barley, B73xCPI-7H 87 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 11 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006 Barley, Lina x H.spont, SNP Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0224 Bmac224
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	Bmac224
Probe for Locus	Bmac224
Locus	Bmac0224 Bmac224
Synonym	Other name Bmac0224
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)5/(AC)5 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.2
Type	PCR SSR
PCR primers	5' GCATATATACCACCCTTGGT 3' 5' ATTCTTGATGGCTATAGCTTG 3'
SSR size	166
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare