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GrainGenes Marker Report: Bmac0303

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Locus	Bmac0303
Type	Microsatellite
Synonym	Synonym Bmac303
Chromosome	5H
Map	Barley, B73xCPI-5H 51.1 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 6 ]
Probe	Bmac0303 Bmac303
Reference	Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Possible Orthologs	Bmac303 Bmac0303a Bmac0303b Bmac0303c Bmac0303e
Probe for Locus	Bmac303
Locus	[ Hide all but 1 of 6 ] Bmac0303 Bmac303 Bmac0303c Bmac0303b Bmac0303e Bmac0303a
Synonym	Other name Bmac0303
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)13(AC)21 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.68
Type	PCR SSR
PCR primers	5' CCTCCAAGATTAGATCTCTCTC 3' 5' CCGTATATTTAAGAAATGGTGA 3'
SSR size	138
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare