GrainGenes Marker Report: Bmac047b-7H
Locus
Bmac047b-7H
Type
Microsatellite
Map
Hv-DxR-Stein-7H 68.88
[ Show Nearby Loci ]
Map Data
Barley, OWB, Stein2006
Probe
Bmac47
Possible Orthologs
[ Hide all but 1 of 8 ] Bmac0047 Bmac47 Bmac0047a Bmac0047b Bmac047B Bmac0047c Bmac0047d Bmac0047e
Probe for Locus
Bmac47
Locus
Bmac0047b
[ Show all 8 ]
Synonym
Other name Bmac0047
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)16 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR SSR
PCR primers
5' AACACACGTACACAAATACACA 3'
5' ACGTCCATCACTTTGACC 3'
SSR size
190
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmac047b-7H
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