GrainGenes Marker Report: Bmac297
Locus
Bmac297
Type
Microsatellite
Synonym
Synonym Bmac0297a Bmac0297c
Probe
Bmac297
Possible Orthologs
Bmac0297a
[ Show all 6 ]
Probe
Bmac297
Locus
[ Hide all but 1 of 7 ] Bmac0297b Bmac0297c Bmac0297a Bmac0297e Bmac0297d Bmac0297f Bmac297
Synonym
Other name Bmac0297
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AC)9(AC)10 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR SSR
PCR primers
5' ATAGAGGGGGTGAAGAATAAC 3'
5' AATAAGTGAATGATGTTGAGGA 3'
SSR size
206
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: Bmac297
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