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GrainGenes Marker Report: Bmag0112

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Locus	Bmag0112
Type	Microsatellite
Synonym	Synonym Bmag112
Chromosome	3H
Map	[ Hide all but 1 of 7 ] Barley-ConsensusFHB-3H 68.14 Hordeum-Consensus2005-SNP-3H 64.233 Hordeum-Consensus2007-SSR-3H 65.5 Hordeum-LiHs-SNP-3H 54.69 Hordeum-LxHs-3H 70 Hordeum-QTLConsensus-Abiotic-3H 64.23 Hordeum-QTLConsensus-Agronomic-3H 64.23
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 7 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0112 Bmag112
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AG)18 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmag112 Bmag0112.3H Bmag0112.7H Bmag112a Bmag112b
Probe for Locus	Bmag112
Locus	Bmag0112 Bmag112 Bmag112a Bmag112b
Synonym	Other name Bmag0112
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)18 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CCCGTGATATATTAAGATCATG 3' 5' AGGGGGAGATCTTCTCTG 3'
SSR size	196
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare