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GrainGenes Marker Report: Bmag0217

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Locus	Bmag0217
Type	Microsatellite
Synonym	Synonym Bmag217
Chromosome	7H
Map	[ Hide all but 1 of 7 ] Barley-ConsensusFHB-7H 77.8 Hordeum-Consensus2005-SNP-7H 83.115 Hordeum-Consensus2007-SSR-7H 79.06 Hordeum-LiHs-SNP-7H 97.29 Hordeum-LxHs-7H 100 Hordeum-QTLConsensus-Abiotic-7H 83.11 Hordeum-QTLConsensus-Agronomic-7H 83.11
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 7 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0217 Bmag217
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AG)19 Polymorphic Information Content (PIC) = 0.69 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmag217
Probe for Locus	Bmag217
Locus	Bmag0217 Bmag217
Synonym	Other name Bmag0217
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)19 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.69
Type	PCR SSR
PCR primers	5' AATGCTCAAATATCTATCATGAA 3' 5' GGGGCTGTCACAAGTATATAG 3'
SSR size	196
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare