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GrainGenes Marker Report: Bmag0222

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Locus	Bmag0222
Type	Microsatellite
Synonym	Synonym Bmag222
Chromosome	5H
Map	Barley, B73xCPI-5H 144.2 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 11 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006 Barley, Lina x H.spont, SNP Barley, LxHs Barley, Nure x Tremois
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0222 Bmag222
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmag222 Bmag222-5H
Probe for Locus	Bmag222
Locus	Bmag0222 Bmag222 Bmag222-5H
Synonym	Other name Bmag0222
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)9(AG)17 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' ATGCTACTCTGGAGTGGAGTA 3' 5' GACCTTCAACTTTGCCTTATA 3'
SSR size	179
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare