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GrainGenes Marker Report: Bmag0353

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Locus	Bmag0353
Type	Microsatellite
Synonym	Synonym Bmag353
Chromosome	4 (4H) 4H
Map	Barley, B73xCPI-4H 64.6 [ Show all 17 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QHei.pil-4H.3 QTgw.pil-4H.3
Map Data	[ Hide all but 1 of 17 ] Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, AxHs/QTL Barley, B73xCPI Barley, Consensus 2005, SNP Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006 Barley, Lx41 Barley, LxHs Barley, OWB Barley, OWB, 2004 Barley, OWB, 2005 Barley, OWB, OPA2008 Barley, OWB, SNP Barley, TxE
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0353 Bmag353
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmag353
Probe for Locus	Bmag353
Locus	Bmag0353 Bmag353
Synonym	Other name Bmag0353
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)21 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.67
Type	PCR SSR
PCR primers	5' ACTAGTACCCACTATGCACGA 3' 5' ACGTTCATTAAAATCACAACTG 3'
SSR size	119
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare