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GrainGenes Marker Report: Bmag0829

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Locus
Bmag0829
Type
Microsatellite
Chromosome
2H
Map
Barley, B73xCPI-2H71.7
Barley-ConsensusFHB-2H73.03
Hordeum-Consensus2007-SSR-2H74.56
Hv-Integrated2009-2H67.3
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Map Data
Barley, B73xCPI
Barley, Consensus 2007, SSR
Barley, Consensus 2023, FHB
Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
Bmag0829
Bmag829
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
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Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex
Insert contains repeat (GA)28
Polymorphic Information Content (PIC) = 0.81
Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmag829



Probe for Locus
Bmag829
Locus
Bmag0829
Bmag829
Synonym
See alsoBmag0829
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (GA)28
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.81
Type
PCR
SSR
PCR primers
5' TAAAAGCCAAACTCGATAATC 3'
5' TTGTTGATGAGAAGTTTTGTG 3'
SSR size
189
Amplification Conditions
3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species
Hordeum vulgare