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GrainGenes Marker Report: Bmag138a

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Locus	Bmag138a
Chromosome	3H
Map	Hv-Consensus2006-Marcel-3H 52.99
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel
Probe	Bmag138
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0138 [ Show all 8 ]
Probe for Locus	Bmag138
Locus	[ Hide all but 1 of 6 ] Bmag0138a Bmag0138b Bmag0138 Bmag138 Bmag138a Bmag138b
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)6(AG)15 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' ACCAGGAGGAATGAGAGAG 3' 5' AATAAACCTTGAGACGATGG 3'
SSR size	169
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare