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GrainGenes Marker Report: CFA2121

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Probe	CFA2121
Locus	[ Hide all but 1 of 9 ] Xcfa2121-2A Xcfa2121-4A Xcfa2121b Xcfa2121-5A Xcfa2121-5B.2 cfa2121 cfa2121-5A Xcfa2121-5B.1 cfa2121-5B
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)20 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TAAATGGCCATCAAGCAATG 3' Reverse 5' GCTTGTGAACTAATGCCTCCC 3'
SSR size	160
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A/2A
Source Species	Triticum urartu