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GrainGenes Marker Report: CFA2158

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Probe	CFA2158
Locus	[ Hide all but 1 of 14 ] Xcfa2158a Xcfa2158b Xcfa2158-1A Xcfa2158-1B Xcfa2158-1D cfa2158 cfa2158-1A cfa2158a cfa2158A-1A cfa2158a-1B cfa2158b cfa2158b-1B cfa2158-1B.1 cfa2158-1B.2
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTTCGTCTTCAAAATGCACTG 3' Reverse 5' TGGTAGCTTACAAAGGTGCG 3'
SSR size	229
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A/1B/1D
Source Species	Triticum urartu