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GrainGenes Marker Report: CFA2185

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Probe	CFA2185
Locus	[ Hide all but 1 of 6 ] Xcfa2185-5D Xcfa2185-5A Xcfa2185.2 Xcfa2185.1 cfa2185 cfa2185-5A
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)29 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTCTTCAGTTGTTTTGGGGG 3' Reverse 5' TTTGGTCGACAAGCAAATCA 3'
SSR size	226
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Triticum urartu