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GrainGenes Marker Report: EBmac0603

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Locus	EBmac0603
Type	Microsatellite
Synonym	Synonym EBmac603
Chromosome	7H
Map	[ Hide all but 1 of 11 ] Barley, B73xCPI-7H 36.2 Barley-ConsensusFHB-7H 38.8 Hordeum-Consensus2005-SNP-7H 59.17 Hordeum-Consensus2007-SSR-7H 35.39 Hordeum-LiHs-SNP-7H 49.81 Hordeum-LxHs-7H 50 Hordeum-QTLConsensus-Abiotic-7H 59.17 Hordeum-QTLConsensus-Agronomic-7H 59.17 Hv-Consensus2006-Marcel-7H 35.39 Hv-Integrated2009-7H 38.3 Hv-Nure/Tremois-7H 32.9
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 11 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0603 EBmac603
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	EBmac603
Probe for Locus	EBmac603
Locus	EBmac0603 EBmac603
Synonym	Other name EBmac0603
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CA)10 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ACCGAAACTAAATGAACTACTTCG 3' 5' TGCAAACTGTGCTATTAAGGG 3' ACCGAAACTAAATGAACTACTTCG TGCAAACTGTGCTATTAAGGG
SSR size	149
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare