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GrainGenes Marker Report: EBmac0701

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Locus	EBmac0701
Type	Microsatellite
Synonym	Synonym EBmac701
Chromosome	4H
Map	Barley, B73xCPI-4H 101 [ Show all 17 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MQTL_Du2024.yield-4H.8
Map Data	Barley, Abiotic QTL Consensus [ Show all 17 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0701 EBmac701
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex,Oregon Wolfe Barleys Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, Steptoe x Morex, OWBrecessive x OWBdominant, Lina x H. spontaneum Insert contains repeat (AC)23 Polymorphic Information Content (PIC) = 0.74 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac0701 Bmac701 EBmac701
Probe for Locus	EBmac701
Locus	EBmac0701 EBmac701
Synonym	Other name Bmac701 EBmac0701
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ATGATGAGAACTCTTCACCC 3' 5' TGGCACTAAAGCAAAAGAC 3'
SSR size	149
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare