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GrainGenes Marker Report: GBM1008

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Locus	GBM1008
Type	Microsatellite
Chromosome	6H
Map	[ Hide all but 1 of 7 ] Barley, B73xCPI-6H 81.8 Barley-ConsensusFHB-6H 92.64 Hordeum-Consensus2007-SSR-6H 95.37 Hordeum-OWB-2004-6H 112.9 Hordeum-OWB-2005-6H 122.2 Hordeum-OWB-OPA2008-6H 118.41 Hv-Integrated2009-6H 95.9
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 7 ]
Species	Hordeum vulgare
Probe	GBM1008
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Probe	GBM1008
Locus	GBM1008 GBM1008-6H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks	Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HY08G04u
Type	SSR EST
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL511537
DNA Library	IPK-HY
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)