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GrainGenes Marker Report: GBM1012

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Locus	GBM1012
Type	Microsatellite
Chromosome	2H
Map	[ Hide all but 1 of 9 ] Barley-ConsensusFHB-2H 149.8 Hordeum-Consensus2007-SSR-2H 149.81 Hordeum-OWB-2004-2H 172.4 Hordeum-OWB-2005-2H 170.1 Hordeum-OWB-OPA2008-2H 182.411 Hv-Consensus2006-Marcel-2H 149.81 Hv-Integrated2009-2H 146.2 Hv-L94/Vada-2006-2H 186.77 Hv-Vada/SusPtrit-2H 135.11
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel [ Show all 9 ]
Species	Hordeum vulgare
Probe	GBM1012
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Probe	GBM1012
Locus	GBM1012 GBM1012-2H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks	Primer sequences for this Microsatellite require a Materials Transfer Agreement (MTA). Those interested should contact Andreas Graner Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW02J20T
Type	PCR SSR EST
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL503561
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)