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GrainGenes Marker Report: GBM1015

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Locus	GBM1015
Type	Microsatellite
Chromosome	4H
Map	Barley, B73xCPI-4H 123.2 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 7 ]
Species	Hordeum vulgare
Probe	GBM1015
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark	[ Hide all but 1 of 8 ] Number of populations contributing to the placement of GBM1015 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex,Oregon Wolfe Barleys,Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, Steptoe x Morex, OWBrecessive x OWBdominant, Igri x Franka Insert contains repeat (ACAT)13 Polymorphic Information Content (PIC) = 0.78 Developing laboratory = IPK (A. Graner)
Probe	GBM1015
Locus	GBM1015 GBM1015-4H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks	Primer sequences for this SSR require a Material Transfer Agreement (MTA). Those interested should contact Andreas Graner Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW04P19u
Type	SSR
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL501672
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)