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GrainGenes Marker Report: GBM1030

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Locus
GBM1030
Type
Microsatellite
Chromosome
7H
Map
Barley-ConsensusFHB-7H77.8
[ Show all 7 ]
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Map Data
[ Hide all but 1 of 7 ]
Barley, Consensus 2006, DArT
Barley, Consensus 2007, SSR
Barley, Consensus 2023, FHB
Barley, Integrated, Marcel 2009
Barley, OWB, 2004
Barley, OWB, 2005
Barley, OWB, OPA2008
Species
Hordeum vulgare
Probe
GBM1030
Reference
ReferenceSzucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
ReferenceThiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark
Number of populations contributing to the placement of GBM1030 in the Barley, Consensus 2006 DArT maps = 1
[ Show all 7 ]



Probe
GBM1030
Locus
GBM1030
GBM1030-7H
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceThiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks
Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner
Clone HW07O20V
Type
SSR
EST
PCR primers
available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL505264
DNA Library
IPK-HW
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)