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GrainGenes Marker Report: GBM1030

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Locus	GBM1030
Type	Microsatellite
Chromosome	7H
Map	Barley-ConsensusFHB-7H 77.8 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT [ Show all 7 ]
Species	Hordeum vulgare
Probe	GBM1030
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark	[ Hide all but 1 of 7 ] Number of populations contributing to the placement of GBM1030 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (AG)17 Polymorphic Information Content (PIC) = 0.44 Developing laboratory = IPK (A. Graner)
Probe	GBM1030
Locus	GBM1030 GBM1030-7H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks	Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW07O20V
Type	SSR EST
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL505264
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)