GrainGenes Marker Report: GBM1053
Locus
GBM1053
Type
Microsatellite
Chromosome
6H
Map
Barley, B73xCPI-1H 60.5 Barley-ConsensusFHB-6H 52.5 Hordeum-Consensus2007-SSR-6H 49.11 Hv-Integrated2009-6H 51.6
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1053
Reference
Remark
[ Hide all but 1 of 6 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex Insert contains repeat (CCG)7 Polymorphic Information Content (PIC) = 0.32 Developing laboratory = IPK (A. Graner)
Probe
GBM1053
Locus
GBM1053 GBM1053-6H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks
Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HY08I23V
Type
SSR
PCR primers
available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL508368
DNA Library
IPK-HY
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1053
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