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GrainGenes Marker Report: GBM1087

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Locus	GBM1087
Type	Microsatellite
Chromosome	6H
Map	Barley-ConsensusFHB-6H 124.99 Hordeum-Consensus2007-SSR-6H 127.7 Hv-Consensus2006-Marcel-6H 127.7 Hv-Integrated2009-6H 129.2 Hv-L94/Vada-2006-6H 130.11
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Map Data	Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006
Species	Hordeum vulgare
Probe	GBM1087
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Oregon Wolfe Barleys Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada Insert contains repeat (AGG)5 Polymorphic Information Content (PIC) = 0.57 Developing laboratory = IPK (A. Graner)
Probe	GBM1087
Locus	GBM1087 GBM1087-6H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HD01H16w
Type	SSR
PCR primers	TAAGATGCACACGTCCAACC TGATACACCTCACAGCTCGG
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ659219
DNA Library	IPK-HD
Source Species	Hordeum vulgare
Source Germplasm	Golden Promise (barley)