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GrainGenes Marker Report: GBM1139

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Locus	GBM1139
Type	Microsatellite
Chromosome	3H
Map	[ Hide all but 1 of 6 ] Barley, B73xCPI-3H 46.1 Barley-ConsensusFHB-3H 57.19 Hordeum-Consensus2007-SSR-3H 52.02 Hv-Consensus2006-Marcel-3H 52.02 Hv-Integrated2009-3H 63.8 Hv-L94/Vada-2006-3H 57.2
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 6 ]
Species	Hordeum vulgare
Probe	GBM1139
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Probe	GBM1139
Locus	GBM1139 GBM1139-3H
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HP01B24w
Type	SSR EST
PCR primers	GCACACACAATGGGAAACAG CGACTAATGAGGGAAGGCAG
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ661747
DNA Library	IPK-HP
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)