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GrainGenes Marker Report: GBM1174

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Locus	GBM1174
Type	Microsatellite
Chromosome	7H
Map	Barley, B73xCPI-7H 93.1 Barley-ConsensusFHB-7H 86.88 Hordeum-Consensus2007-SSR-7H 93.86 Hv-Integrated2009-7H 95.1
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Map Data	Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1174
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 6 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex Insert contains repeat (CGG)6 Polymorphic Information Content (PIC) = 0.24 Developing laboratory = IPK (A. Graner)
Probe	GBM1174
Locus	GBM1174 GBM1174-7H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HU01G21T
Type	SSR
PCR primers	Forward TCTGGAAGAGGAAGGTGAGC Reverse TTCCTCTTACCGTTCTTCGC TCTGGAAGAGGAAGGTGAGC TTCCTCTTACCGTTCTTCGC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ464777
DNA Library	IPK-HU
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)