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GrainGenes Marker Report: GBM1176

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Locus	GBM1176
Type	Microsatellite
Chromosome	5H
Map	[ Hide all but 1 of 6 ] Barley, B73xCPI-5H 23.7 Barley-ConsensusFHB-5H 33.65 Hordeum-Consensus2007-SSR-5H 18.59 Hv-Consensus2006-Marcel-5H 18.59 Hv-Integrated2009-5H 21.2 Hv-L94/Vada-2006-5H 17.76
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 6 ]
Species	Hordeum vulgare
Probe	GBM1176
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Probe	GBM1176
Locus	GBM1176 GBM1176-5H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HU01M19w
Type	SSR EST
PCR primers	Forward TATACATCAGCGGGCCTTTT Reverse CTCCAACCTCGCAAAGAGTC TATACATCAGCGGGCCTTTT CTCCAACCTCGCAAAGAGTC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ663268
DNA Library	IPK-HU
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)