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GrainGenes Marker Report: GBM1187

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Locus
GBM1187
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H16.85
Hordeum-Consensus2007-SSR-2H19.51
Hv-Consensus2006-Marcel-2H19.51
Hv-Integrated2009-2H17.1
Hv-L94/Vada-2006-2H5.52
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel
Barley, Consensus 2007, SSR
Barley, Consensus 2023, FHB
Barley, Integrated, Marcel 2009
Barley, L94 x Vada, 2006
Species
Hordeum vulgare
Probe
GBM1187
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark
[ Hide all but 1 of 7 ]
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, Steptoe x Morex
Insert contains repeat (CCG)5
Polymorphic Information Content (PIC) = 0.49
Developing laboratory = IPK (A. Graner)



Probe
GBM1187
Locus
GBM1187
GBM1187-2H
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner
Clone HV01H01T
Type
SSR
PCR primers
GCCAAGAGCATCAAGAGGAT
GTGGGGACTATGTCCGGC
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ471178
DNA Library
IPK-HV
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)