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GrainGenes Marker Report: GBM1221

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Locus	GBM1221
Type	Microsatellite
Chromosome	4H
Map	Barley, B73xCPI-4H 8.4 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	[ Hide all but 1 of 7 ] Barley, B73xCPI Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006
Species	Hordeum vulgare
Probe	GBM1221
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	Number of populations contributing to the placement of GBM1221 in the Barley, Consensus 2006 DArT maps = 1 [ Show all 8 ]
Probe	GBM1221
Locus	GBM1221 GBM1221-4H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HW02L22u
Type	SSR EST
PCR primers	ACCAGCAATCCAAGTTACGG TGCCTTGGTCTTGGTGTGTA Forward ACCAGCAATCCAAGTTACGG Reverse TGCCTTGGTCTTGGTGTGTA
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL501017
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)