GrainGenes Marker Report: GBM1300
Locus
GBM1300
Type
Microsatellite
Chromosome
3H
Map
Barley, B73xCPI-3H 42.1 Barley-ConsensusFHB-3H 53.55 Hordeum-Consensus2007-SSR-3H 47.34 Hv-Integrated2009-3H 52.6
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1300
Reference
Remark
[ Hide all but 1 of 6 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (ATC)6 Polymorphic Information Content (PIC) = 0.24 Developing laboratory = IPK (A. Graner)
Probe
GBM1300
Locus
GBM1300 GBM1300-3H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HV03G09u
Type
SSR
PCR primers
TGGATGCTCCTCCCAATTTA
ATGCATGTTGTGCCCAAGTA
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ664456
DNA Library
IPK-HV
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1300
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