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GrainGenes Marker Report: GBM1334

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Locus	GBM1334
Type	Microsatellite
Chromosome	1H 7H
Map	Barley-ConsensusFHB-1H 72.47 Barley-ConsensusFHB-7H 77.8 Hordeum-Consensus2006-DArT-7H 74.7 Hordeum-Consensus2007-SSR-1H 70.69 Hv-Integrated2009-1H 71.5
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Map Data	Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1334
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 7 ] Number of populations contributing to the placement of GBM1334 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (GGC)8 Polymorphic Information Content (PIC) = 0.49 Developing laboratory = IPK (A. Graner)
Probe	GBM1334
Locus	GBM1334.1H GBM1334.7H GBM1334 GBM1334-1H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HV01J13T
Type	SSR
PCR primers	CCTCTTCCGAATCCATCAAA GAGTTGGGGACTTTGCTGAC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ471211
DNA Library	IPK-HV
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)