GrainGenes Marker Report: GBM1421
Locus
GBM1421
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H 156.37 Hordeum-Consensus2007-SSR-2H 156.38 Hv-Integrated2009-2H 153
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1421
Reference
Remark
[ Hide all but 1 of 6 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (ACACAA)5 Polymorphic Information Content (PIC) = 0.49 Developing laboratory = IPK (A. Graner)
Probe
GBM1421
Locus
GBM1421 GBM1421-2H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HD03K15u
Type
SSR
PCR primers
TGTCTGTTTCAATCCATTCCAG
TGCAGTTACTAATCCGCGTG
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ658753
DNA Library
IPK-HD
Source Species
Hordeum vulgare
Source Germplasm
Golden Promise (barley)
GrainGenes Marker Report: GBM1421
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