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GrainGenes Marker Report: GBM1436

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Locus	GBM1436
Type	Microsatellite
Chromosome	5H
Map	Barley, B73xCPI-5H 120.1 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 7 ]
Species	Hordeum vulgare
Probe	GBM1436
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 8 ] Number of populations contributing to the placement of GBM1436 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, Steptoe x Morex Insert contains repeat (CGC)7 Polymorphic Information Content (PIC) = 0.57 Developing laboratory = IPK (A. Graner)
Probe	GBM1436
Locus	GBM1436 GBM1436-5H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HA11A03r
Type	SSR EST
PCR primers	ACGAGGCAAAGGTAGGGTTT ACTTGATGAAGCCGATGTCC Forward ACGAGGCAAAGGTAGGGTTT Reverse ACTTGATGAAGCCGATGTCC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BU977197
DNA Library	IPK-HA
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)