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GrainGenes Marker Report: GBM1470

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Locus	GBM1470
Type	Microsatellite
Chromosome	5H
Map	Barley, B73xCPI-5H 51.1 Barley-ConsensusFHB-5H 63.13 Hordeum-Consensus2006-DArT-5H 57 Hordeum-Consensus2007-SSR-5H 130.55 Hv-Integrated2009-5H 129.4
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Map Data	Barley, B73xCPI Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1470
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 7 ] Number of populations contributing to the placement of GBM1470 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (TC)9 Polymorphic Information Content (PIC) = 0.49 Developing laboratory = IPK (A. Graner)
Probe	GBM1470
Locus	GBM1470 GBM1470-5H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HF12M14r
Type	SSR
PCR primers	Forward TTTCCAGTCAGTCAGTCCCA Reverse ACTTTTGATGGACAAACCGC TTTCCAGTCAGTCAGTCCCA ACTTTTGATGGACAAACCGC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BU986745
DNA Library	IPK-HF
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)