GrainGenes Marker Report: GBM1475
Locus
GBM1475
Type
Microsatellite
Chromosome
2H
Map
[ Hide all but 1 of 6 ] Barley-ConsensusFHB-2H 149.46 Hordeum-Consensus2007-SSR-2H 149.47 Hv-Consensus2006-Marcel-2H 149.47 Hv-Integrated2009-2H 145.9 Hv-L94/Vada-2006-2H 186.69 Hv-Vada/SusPtrit-2H 135.05
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel
[ Show all 6 ]
Species
Hordeum vulgare
Probe
GBM1475
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 7 ]
Probe
GBM1475
Locus
GBM1475 GBM1475-2H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HI05P24u
Type
SSR
PCR primers
CAACTACCAATATCGCCCGT
AGACAGACCCATCCCTCCTT
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ660901
DNA Library
IPK-HI
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1475
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