GrainGenes Marker Report: GBM1475
Locus
GBM1475
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H 149.46
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel
[ Show all 6 ]
Species
Hordeum vulgare
Probe
GBM1475
Reference
Remark
[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L94 x Vada, Vada x SusPtrit, Steptoe x Morex, Oregon Wolfe Barleys Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Vada x SusPtrit; Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : L94 x Vada, SusPtrit x Vada, Steptoe x Morex, OWBrecessive x OWBdominant Insert contains repeat (CTCA)5n(TC)8 Polymorphic Information Content (PIC) = 0.61 Developing laboratory = IPK (A. Graner)
Probe
GBM1475
Locus
GBM1475 GBM1475-2H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HI05P24u
Type
SSR
PCR primers
CAACTACCAATATCGCCCGT
AGACAGACCCATCCCTCCTT
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ660901
DNA Library
IPK-HI
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1475
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