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GrainGenes Marker Report: GBM1501

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Locus	GBM1501
Type	Microsatellite
Chromosome	4H
Map	Barley-ConsensusFHB-4H 0 Hordeum-Consensus2007-SSR-4H 0 Hv-Integrated2009-4H 6.1
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1501
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	[ Hide all but 1 of 6 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant Insert contains repeat (TAGA)6 Polymorphic Information Content (PIC) = 0.41 Developing laboratory = IPK (A. Graner)
Probe	GBM1501
Locus	GBM1501 GBM1501-4H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HS08I08u
Type	SSR
PCR primers	Forward TGTGGACAGTCAAACACGCT Reverse AAGGAGCACCTGAAAAGCAA TGTGGACAGTCAAACACGCT AAGGAGCACCTGAAAAGCAA
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	CA000412
DNA Library	IPK-HS
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)