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GrainGenes Marker Report: HVM36

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Locus	HVM36
Type	Microsatellite
Synonym	Synonym HVM36
Chromosome	2 (2H) 2H
Chromosome Arm	2HS
Map	Barley-consensus-2003-2H 11.5 [ Show all 18 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.DON_2H_2 MetaQ.FHB_2H_1 QFhs.umn-2H.1
Map Data	[ Hide all but 1 of 18 ] Barley consensus 2003 Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, Cebada Capa x SusPtrit Barley, Consensus 2005, SNP Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, HN x H602, Sato2009 Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006 Barley, Lina x H.spont, SNP Barley, Lx41 Barley, LxHs Barley, Steptoe x Morex, SNP Barley, TxE
Species	Hordeum spontaneum Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	HVM36 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. [ Show all 6 ]
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of HVM36 in the Barley, Consensus 2006 DArT maps = 3 [ Show all 8 ]
Probe	HVM36
Locus	HVM36 HVM0036
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (GA)13.
Type	PCR SSR
PCR primers	5' TCCAGCCGAACAATTTCTTG 3' 5' AGTACTCCGACACCACGTCC 3' TCCAGCCGAACAATTTCTTG AGTACTCCGACACCACGTCC TCCAGCCGACAATTTCTTGHvGLUEND PCR_primers TCCAGCCGACAATTTCTTGscssr10226 PCR_primers
SSR size	0.114
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC. SSR_size
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.