Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: HVM67

[Submit comment/correction]

Locus	HVM67
Type	Microsatellite
Synonym	Correct name HVM67 Other name HVM67 Synonym Hsh (Hordeum)
Chromosome	4 (4H) 4H
Chromosome Arm	4HL
Map	Barley-consensus-2003-4H 102 [ Show all 23 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QFhs.HaTu-4H QHea.pil-4H.4
Map Data	Barley consensus 2003 [ Show all 23 ]
Species	Hordeum spontaneum Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	HVM67 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Associated Gene	Hsh (Hordeum)
Gene Class	Hairy leaf sheath
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. [ Show all 8 ]
Data Source	Kleinhofs, Andris Langridge, Peter J.
Remark	[ Hide all but 1 of 9 ] Number of populations contributing to the placement of HVM67 in the Barley, Consensus 2006 DArT maps = 1 This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys,Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Oregon Wolfe Dom x Rec; Igri x Franka Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant, Igri x Franka, Lina x H. spontaneum Insert contains repeat (GA)11 Polymorphic Information Content (PIC) = 0.53 Developing laboratory = VPISU (MA Saghai Maroof)
Gene Associated with Locus	Hsh (Hordeum)
Full Name	Hairy leaf sheath
Synonym	Synonym Hs (Hordeum) von Wettstein-Knowles P (1993) Barley (Hordeum vulgare) 2N = 14. Genetic maps: Locus Maps of Complex Genomes.
Gene Class	Hairy leaf sheath
Allele	Hsh (Hordeum) hsh (Hordeum)
Locus	Hsh HVM67
Chromosome	4H
Image	Barley, hsh (hairless) phenotype Barley, Hsh (hairy leaf sheath) phenotype
Data Source	Franckowiak, Jerome D. 96.10
Probe	HVM67
Locus	HVM67 HVM67-4H hvm67a hvm67b
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (GA)11.
Type	PCR SSR
PCR primers	5' GTCGGGCTCCATTGCTCT 3' 5' CCGGTACCCAGTGACGAC 3' GTCGGGCTCCATTGCTCT CCGGTACCCAGTGACGAC
SSR size	0.116
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.