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GrainGenes Marker Report: HVM74

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Locus	HVM74
Type	Microsatellite
Synonym	Correct name HVM074 Synonym HVM74
Chromosome	6H
Chromosome Arm	6HS
Map	[ Hide all but 1 of 12 ] Barley-consensus-2003-6H 18.8 Barley-ConsensusFHB-6H 59.89 Hordeum-Consensus2005-SNP-6H 70.683 Hordeum-Consensus2007-SSR-6H 62.66 Hordeum-Graner2-6H 66 Hordeum-Lx41-6H 0 Hordeum-QTLConsensus-Abiotic-6H 70.68 Hordeum-QTLConsensus-Agronomic-6H 70.68 Hordeum-StMo-SNP-6H 61.74 Hv-Consensus2006-Marcel-6H 62.66 Hv-Integrated2009-6H 61.1 Hv-L94/Vada-2006-6H 60.75
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 [ Show all 12 ]
Species	Hordeum vulgare
Probe	HVM74 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Data Source	Langridge, Peter J.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Probe	HVM74
Locus	HVM74 HVM0074 HVM074
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (GA)13.
Type	PCR SSR
PCR primers	5' AGGAAGTCATTGCGTGAG 3' 5' TGATCAAGAATGATAACATGG 3' AGGAAGTCATTGCGTGAG TGATCAAGAATGATAACATGG
SSR size	0.162
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.