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GrainGenes Marker Report: HVSS1

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Locus
HvSS1
Type
Microsatellite
Chromosome
7H
Map
Barley-consensus-2003-7H78.6
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Map Data
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Barley consensus 2003
Barley, AxHs/QTL
Barley, Consensus 2006, DArT
Barley, Consensus 2023, FHB
Barley, IxF
Barley, Lx41
Probe
HVSS1
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
ReferencePillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Data Source
Langridge, Peter J.
Remark
Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Number of populations contributing to the placement of HvSS1 in the Barley, Consensus 2006 DArT maps = 1


Probe
HVSS1
Locus
HVSS0001
HvSS1
Reference
ReferenceHayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferencePillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks
Insert contains repeat (t)20
Type
SSR
PCR primers
Forward TGGCCTTGGTGAATGTTTCG
Reverse TGTGGAGGCGAGTCAGCTTG
Left 5' TGGCCTTGGTGAATGTTTCG
Right 5' TGTGGAGGCGAGTCAGCTTG
TGGCCTTGGTGAATGTTTCG
TGTGGAGGCGAGTCAGCTTG
SSR size
240
Amplification Conditions
Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species
Hordeum vulgare