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GrainGenes Marker Report: HvB23D4

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Locus	HvB23D4
Type	Microsatellite
Chromosome	4H
Map	Hordeum-Consensus2006-DArT-4H 38.8 Hordeum-Pillen-4H 26
	[ Show Nearby Loci ]
QTL with Locus Marker	[ Hide all but 1 of 6 ] QHea.pil-4H.2 QHei.pil-4H.2 QHi.pil-4H.2 QPro.pil-4H QTgw.pil-4H.2 QWa.pil-4H.2
Map Data	Barley, AxHs/QTL Barley, Consensus 2006, DArT
Probe	HvB23D4
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of HvB23D4 in the Barley, Consensus 2006 DArT maps = 1
Probe	HvB23D4
Locus	HvB23D4
Synonym	Other name HvOLE1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
Remarks	Insert contains repeat(GCCT)4 SSR size deduced from sequence: 198 bp
Type	PCR
PCR primers	5' GATGGATGTCAGTCGGTC 3' ATGAGCAGTAGTACAACTCTAAGC Forward GATGGATGTCAGTCGGTC Reverse ATGAGCAGTAGTACAACTCTAAGC
Amplification Conditions	40 cycles touchdown at 94, 64-55, 72 C
Sequence	X76603
Source Gene Class	Oleosin
Source Species	Hordeum vulgare