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GrainGenes Marker Report: Sr28

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Locus	Sr28
Type	Gene
Chromosome	2B
Chromosome Arm	2BL
Map	Triticum-Genes-2B 39.6 Wheat-2014-Consensus-Ug99-Update_2B 115
	[ Show Nearby Loci ]
Map Data	Wheat genes Wheat-2014-Consensus-Ug99-Update
2 Point Data	Centromere/Sr28#1 Distance: 34.6 cM Lr23/Sr28#1 Distance: 28.5 cM Sr28/Sr16#1 Distance: 29.2 cM Sr28/Sr16#2 Distance: 38.2 cM Sr9b/Sr28#1 Distance: 16.8 cM
Probe	WMC332 wPt-7004
Associated Gene	Sr28 (Triticum)
Gene Class	Reaction to Puccinia graminis Pers.
Reference	Yu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581. Rouse MN et al. (2012) Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.) Theoretical and Applied Genetics 125:877-885. McIntosh RA (1978) Cytogenetical studies in wheat. X. Monosomic analysis and linkage studies involving genes for resistance to Puccinia graminis f.sp. tritici in cultivar Kota. Heredity 41:71-82. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr28 MAS Wheat. Marker Assisted Selection in Wheat.
Remark	The location on the 2014 Ug99 Consensus Map is approximate.
Probe for Locus	WMC332
Locus	[ Hide all but 1 of 6 ] Xwmc332-2B Xwmc332 wmc332-2B Sr28 wmc332 WMC332
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b12 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2B Trimed to insert at both ends. (CT)12 136 to 159.
Type	PCR SSR
PCR primers	Forward cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65 Reverse gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC332
Source Species	Triticum aestivum