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GrainGenes Marker Report: Sr9a

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Locus
Sr9a
Type
Trait
Chromosome
2B
Chromosome Arm
2BL
Map
Wheat-2014-Consensus-Ug99-Update_2B105
Wheat-Composite2004-2B101
[ Show Nearby Loci ]
Map Data
Wheat-2014-Consensus-Ug99-Update
Species
Triticum aestivum
Probe
WMS47
Associated Gene
Sr9 (Triticum)
Gene Class
Reaction to Puccinia graminis Pers.
Reference
ReferenceYu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581.
ReferenceTsilo TJ et al. (2007) Microsatellite Markers Linked to Stem Rust Resistance Allele Sr9a in Wheat Crop Science 47:2013-2020.
Remark
Trait marker Xgwm47, Xwmc175
Nearby marker on Composite map: Xgwm47
Position on Composite map estimated by GrainGenes.
The location on the 2014 Ug99 Consensus Map is approximate.


Probe for Locus
WMS47
Locus
[ Hide all but 1 of 16 ]
Xgwm47-2A.2
Xgwm47-2A.1
Xgwm47-2B
Xgwm47-5A
Xgwm47.3
Xgwm47.1
Xgwm47.4
Xgwm47.2
Xgwm47b
Xgwm47
Xgwm47-2A
Sr9a
gwm47
gwm47-2A
gwm47-2B
gwm47-5A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
ReferenceRoeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks
Insert contains repeat (CT)7TT(CT)16
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Repeats type (CT)x7TT(CT)x16.
Product size 166 bp in Chinese Spring.
Type
PCR
SSR
PCR primers
5' TTGCTACCATGCATGACCAT 3'
5' TTCACCTCGATTGAGGTCCT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS47
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring